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KMID : 0624620130460110550
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BMB Reports
2013 Volume.46 No. 11 p.550 ~ p.554
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miR-15b induced by platelet-derived growth factor signaling is required for vascular smooth muscle cell proliferation
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Kim Sung-Hwan
Kang Ha-Ra
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Abstract
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The platelet-derived growth factor (PDGF) signaling pathway is essential for inducing a dedifferentiated state of vascular smooth muscle cells (VSMCs). Activation of PDGF inhibits smooth muscle cell (SMC)-specific gene expression and increases the rate of proliferation and migration, leading to dedifferentiation of VSMCs. Recently, microRNAs have been shown to play a critical role in the modulation of the VSMC phenotype in response to extracellular signals. However, little is known about microRNAs regulated by PDGF in VSMCs. Herein, we identify microRNA-15b (miR-15b) as a mediator of VSMC phenotype regulation upon PDGF signaling. We demonstrate that miR-15b is induced by PDGF in pulmonary artery smooth muscle cells and is critical for PDGF-mediated repression of SMC-specific genes. In addition, we show that miR-15b promotes cell proliferation. These results indicate that PDGF signaling regulates SMC-specific gene expression and cell proliferation by modulating the expression of miR-15b to induce a dedifferentiated state in the VSMCs.
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KEYWORD
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Cell proliferation, Dedifferentiation, microRNA, PDGF, Vascular smooth muscle cell
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